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WebApr 30, 2024 · THP-1 cells (5-6 × 10 6 cells/ml) were labeled with 10 μM calcein AM (Sigma-Aldrich, USA) for 45 min at 37° C. After the stimulation, THP-1 cells were added to HAECs at a ratio of 5:1 and incubated for 2 h at 37° C in darkness. Unbound cells … WebFeb 25, 2024 · Hence, the optimal seeding density of THP-1 cell line that would be used all throughout this project was 2.5×10^5 cells/mL. There are multiple AML studies using THP-1 as cellular model, and the seeding densities vary from low values such as 0.5×10^5 cells/mL to higher values such as 5×10^5 cells/mL (Jagan et al., 2012). WebDonate. THEP enables hilltribe children from remote and marginalised areas to: 1. Further their education and develop the knowledge and skills necessary to reach their goals. 2. … margaret josephs company logo