WebProduct Information. 29414-1-AP targets F4/80 in IHC, IF, ELISA applications and shows reactivity with mouse samples. EGF-like module containing, mucin-like, hormone receptor-like sequence 1. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C. WebMay 20, 2008 · Here we have optimised a triple colour immunofluorescent staining protocol, utilising an anti-FITC technique, to allow antibodies to macrophage markers to be used simultaneously. We highlight the substantial heterogeneity of cells in both normal liver and spleen that stain for F4/80, CD68, CD11b, and CD11c.
F4/80 Antibody Staining Protocol for Immunohistochemistry - IHC …
WebFluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). WebApr 9, 2024 · The tumor suspensions were marked with CD11b-FITC, F4/80-APC, CD16/32-PerCP/cyanine 5.5, and CD206-BV421 antibodies to evaluate tumor-associated macrophages (TAMs). To detect T-cells, spleen suspensions were incubated with CD3-PerCP/cyanine 5.5, CD4-FITC, CD8-APC, CD69-PE/cyanine7, IFN-γ-PE, and granzyme … openmct yamcs
FITC anti-mouse F4/80 Recombinant Antibody - BioLegend
WebPrimary Antibody. Name: F4/80 Antibody. Clone: CI:A3-1, Rat anti-Mouse. Supplier: Caltag Labs (Invitrogen) Catalog Number: RM290. Dilution: 1:50 using IHC-Tek TM Antibody … WebJul 23, 2012 · Flow Cytometry - FITC Anti-F4/80 antibody [CI:A3-1] (ab105155) Flow cytometry staining of mouse peritoneal macrophages with ab105155. FSC/SSC gated mouse peritoneal macrophages dual stained … WebThe F4/80 antigen is expressed by a majority of mature macrophages and is one of the best markers for this population of cells. However, other cell types, such as peritoneal … ipad deals at currys